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	Studie antimikrobiálního účinku včelího jedu na druhy borelie z komplexu \kur{B. burgdorferi} sensu lato: izolace genu kódujícího antimikrobiální peptid melitin z jedové žlázy včely medonosné \kur{(Apis mellifera)} DAVIDOVÁ, Marie The incidence of tick borne diseases has drastically increased over the past few years. Control of tick borne pathogens, especially spirochetes from \kur{B. bugdorferi} sensu lato complex, is complicated due to a lack of vaccines against them. The emergence of bacterial strains to well established and widely used antibiotics has become a serious public health concern. Analysis of new classes of antimicrobial peptides became the subject of new investigation due to their efficacy against disease causing pathogens. Among possible candidates, melittin from honey bee venom is of particular interest. The aim of this work was to study the effect of whole bee venom on Lyme borreliosis spirochetes. The minimum concentration of the venom that possesed the inhibitory effect on borrelia growth was identified. The gene enconding melittin was isolated from bee venom gland and recombinant melittin was produced in bacterial expression system. The effect of recombinant melittin on live borrelia culture was studied. Detailed record
	Evolution of peptides with an antimicrobial activity in venom of bees from genus Nomada (Hymenoptera: Apoidea) Matějková, Zuzana ; Straka, Jakub (advisor) ; Šobotník, Jan (referee) Detailed record
	Evolution of sting and associated venom gland in Hymenoptera (Aculeata) Matějková, Zuzana ; Straka, Jakub (advisor) ; Šobotník, Jan (referee) Theme: Evolution of the sting and associated glands in Hymenoptera (Aculeata) Author: Zuzana Matějková Supervisor: Mgr. Jakub Straka, Ph.D. Abstract A stinger is a synapomorphy of Aculeata group. The branch of Aculeata has cleaved from the Parasitica group - they both belong to the order Apocrita. The stinger of Aculeata has evolved from a former ovipositor - though the function of laying eggs has been lost. Besides venom gland several other glands including Dufour's gland vent into a stinger. The original (parasitic-predator strategy- binding) function of the gland excretes is a paralysis of the prey. In social Aculeata it gains a defensive function - associated with a possible act of autotomy of the stinger. The stinger and associated glands are also a source of pheromones and small peptides with an antimicrobial function. These peptides act together with other venom components and they help to improve its effectiveness. Detailed record

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